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The Monoclonal Antibody Unit offers a reliable, speedy, and economical service for your custom polyclonal and monoclonal antibody needs.
(1) Our service provides you:
Mouse Monoclonal Antibody Generation
- Isolated spleen cells from immunized mouse (for immunization service, please refer to BRC)
- Fusion of Spleen Cells with Sp2/0 Myeloma Cells
- ELISA Screening
- Western Blot Screening
- Indirect Immunofluorescent Staining of Cells
- Immunoglobulin Isotyping
- Flow cytometry
- Immunoglobulin Isotyping
- Subcloning and Rescuing Unstable Hybridoma Clones
- Ascites Production
The service is open to all researchers in Singapore.
Please following this link for estimated charges.
For more information on using the service,
please contact IMCB Joint PI, Dr Tan Yee Joo, at
mcbtanyj@imcb.a-star.edu.sg
or go to: https://corefac.imcb.a-star.edu.sg/imcbmau/
2) Our workflow and equipments
a) Work Flow for Mouse Monoclonal Antibody Production
Please click on the image above to view larger image (pdf format)
b) Automation of Cell Confluency Detection, Well Re-arrangement,
Supernatant Collection and ELISA
The Player show be shown here
Click the "Play" icon to view the video.
c) Automation of Cell Staining Using DropArray Technology
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(3) Recent Commercialized mAbs which were produced by MAU
Antigen |
Requestor |
Application |
Alpha C |
NNI |
WB, IF, ELISA |
Gamma C |
NNI |
WB, IF, ELISA |
SGT |
CAVR |
WB, IP, IF, ELISA |
MOAP-1-N |
VY |
WB, IP, IF, ELISA |
Bam32 |
LKP |
WB, IP, ELISA |
SCL-C |
WZL |
WB, IF, ELISA |
SCL-N |
WZL |
WB, IF, ELISA |
beta 3 |
WH |
WB, IP, FACS, IF, ELISA |
cld 16 |
WH |
IF, ELISA |
SNX27 |
HWJ |
WB, IP, IF, ELISA |
TOM1L1 |
HWJ |
WB, IP, IF, ELISA |
Fish mib |
JYJ |
WB, IF, ELISA |
P53 |
DPL |
WB, IF, ELISA |
AIF |
AGP |
WB, IF, ELISA |
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